Quantitative real-time PCR tests for diagnostic and prognostic purposes in cases of legionellosis
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چکیده
منابع مشابه
Legionellosis and Lung Abscesses: Contribution of Legionella Quantitative Real-Time PCR to an Adapted Followup
We report a case of severe Legionnaires' disease (LD) complicated by a lung abscess in an immunocompetent patient who required ECMO therapy and thoracic surgery. The results of repeated Legionella quantitative real-time PCR performed on both sera and respiratory samples correlated with the LD severity and the poor clinical outcome. Moreover, the PCR allowed for the detection of Legionella DNA i...
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Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2010
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2009.02812.x